2004年9月15日

For calculating Hydrogen Bond

HBPLUS, which is distributed with LIGPLOT: http://www.biochem.ucl.ac.uk/bsm/ligplot/ligplot.html

There's also HBOND, which comes with the JOY package:
http://www-cryst.bioc.cam.ac.uk/~joy/

they are free, but they all need Academic License which I can't apply individually, has to apply by UMDNJ.

for example, I want to know the H-Bonds in protein 1a2p, I go to

http://bioportal.weizmann.ac.il/oca-bin/csu?PDB_ID=1a2p

I found there's four sheet strands for each chain.
I click on "Strand 1" on "A sheet" (residue 96-99)
I'll go to this page
from this page, if there's a "+" sign under HB colume, I know there's a H-Bond.

2004年9月5日

2004年8月15日

Fixed the input problem (sometimes it's in Chinese input mode, but don't let me input Chinese)
it's because I hit the Shift key, it changes to temporary English mode, but shows it's in Chinese mode. (If you show the input bar in system bar, you'll find the difference.)

web folder can be easily setup if you use IIS server, just create a folder and done.
But, because my tablet use FAT32 format, I can't set permission for files.

2004年8月3日

use
http://eutils.ncbi.nlm.nih.gov/entrez/eutils/soap/eutils.wsdl
to check pubmed data

ex:
Feasibility of simplifying balloon mitral valvuloplasty by obviating left-sided cardiac catheterization using on-line guidance with transesophageal echocardiography.
PMID: 12796174

1. use run_eSearch
db=pubmed
term=Feasibility+of+simplifying+balloon+mitral+valvuloplasty
field=titl
you'll get PMID: 12796174
http://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi?db=pubmed&term=Feasibility+of+simplifying+balloon+mitral+valvuloplasty&field=titl

2.use run_eSummary
db=pubmedid=12796174
you'll get:
Feasibility of simplifying balloon mitral valvuloplasty by obviating left-sided cardiac catheterization using on-line guidance with transesophageal echocardiography.
http://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&id=12796174&retmode=xml

2004年7月22日

Use this to calculate ANOVA
http://lgsun.grc.nia.nih.gov/ANOVA/bin/login.cgi
http://bioinformatics.uams.edu/page/

Use this to translate gene identifier
http://discover.nci.nih.gov/matchminer/html/MatchMinerInteractiveLookup.jsp

Use EXCEL to add color to updowngene.txt and use this to get it in pathway (colored)
http://www.genome.ad.jp/kegg-bin/mk_point_multi_html

I'm thinking about use pathway data and microarray data to form an automatic functional genetic discoverer. (use the same algorithm I do for Dr. Gu)


2004年7月13日

for researcher M.S.Chang
these tools might help.

ex:
String http://string.embl.de/
input sequence, get "predict functional associations proteins/genes"
supported by microarray, textmining, Co-occurrence, etc.

IntAct http://www.ebi.ac.uk/intact/index.html
input gene, it will give you proteins interact with the given gene.
supported by Experiments.

InterDom
input sequence, it will give you putative domain interactions
for example, it will give you some domains which has complexes with domain in given sequence (derived from PDB), or has protein interactions with domain in given sequence (derived from DIP).

after visit Mackay memorial hospital (in Taiwan)
I done some research on Database of Interacting Proteins (DIP)
ex: I want to know what proteins interact with P53
enter P53 in NodeID or Name field in this page
you will get DIP nodes for p53 in human and mouse
(DIP:368N, DIP:369N, DIP:5978N, DIP:24202N, etc)
in each DIP node, you can click on the "graph" link at top right corner
you'll see what other proteins interact with P53.

it also provides links to related information in
PDB, NCBI, Prosite, Smart, InterPro databases.

another one similar to this